j774 llc mk 2 Search Results


j774 llc mk 2  (ATCC)
99
ATCC j774 llc mk 2
J774 Llc Mk 2, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
j774 llc mk 2 - by Bioz Stars, 2026-03
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primary human fibroblasts nigms  (Coriell Institute for Medical Research)
90
Coriell Institute for Medical Research primary human fibroblasts nigms
Ionomycin induces exocytosis of β-hexosaminidase and lysosomal fluid-phase tracers but not LDH from intact NRK <t>fibroblasts.</t> ( a and b ) NRK cells were incubated at 37°C with either PBS ( open circles ) or 10 μM ionomycin in PBS ( black circles ). At the indicated time points, the incubation buffer was collected and assayed for β-hexosaminidase and LDH activity. The amount of enzyme released at each point is expressed as a percentage of the total content of enzyme in control cells. ( c and d ) Lysosomes of NRK cells were loaded with lucifer yellow or 3 H-dextran by fluidphase endocytosis followed by a 2-h chase, before treatment with PBS or 10 μM ionomycin for 10 min. The incubation buffer was collected, lucifer yellow was detected by reading the fluorescence, and 3 H-dextran was detected by scintillation counting. The amount of lucifer yellow or 3 H-dextran released in each sample is expressed as a percentage of the total amount of tracer present in control cells. The data represent the average of triplicate determinations ±SD.
Primary Human Fibroblasts Nigms, supplied by Coriell Institute for Medical Research, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary human fibroblasts nigms/product/Coriell Institute for Medical Research
Average 90 stars, based on 1 article reviews
primary human fibroblasts nigms - by Bioz Stars, 2026-03
90/100 stars
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Ionomycin induces exocytosis of β-hexosaminidase and lysosomal fluid-phase tracers but not LDH from intact NRK fibroblasts. ( a and b ) NRK cells were incubated at 37°C with either PBS ( open circles ) or 10 μM ionomycin in PBS ( black circles ). At the indicated time points, the incubation buffer was collected and assayed for β-hexosaminidase and LDH activity. The amount of enzyme released at each point is expressed as a percentage of the total content of enzyme in control cells. ( c and d ) Lysosomes of NRK cells were loaded with lucifer yellow or 3 H-dextran by fluidphase endocytosis followed by a 2-h chase, before treatment with PBS or 10 μM ionomycin for 10 min. The incubation buffer was collected, lucifer yellow was detected by reading the fluorescence, and 3 H-dextran was detected by scintillation counting. The amount of lucifer yellow or 3 H-dextran released in each sample is expressed as a percentage of the total amount of tracer present in control cells. The data represent the average of triplicate determinations ±SD.

Journal: The Journal of Cell Biology

Article Title: Lysosomes Behave as Ca 2+ -regulated Exocytic Vesicles in Fibroblasts and Epithelial Cells

doi:

Figure Lengend Snippet: Ionomycin induces exocytosis of β-hexosaminidase and lysosomal fluid-phase tracers but not LDH from intact NRK fibroblasts. ( a and b ) NRK cells were incubated at 37°C with either PBS ( open circles ) or 10 μM ionomycin in PBS ( black circles ). At the indicated time points, the incubation buffer was collected and assayed for β-hexosaminidase and LDH activity. The amount of enzyme released at each point is expressed as a percentage of the total content of enzyme in control cells. ( c and d ) Lysosomes of NRK cells were loaded with lucifer yellow or 3 H-dextran by fluidphase endocytosis followed by a 2-h chase, before treatment with PBS or 10 μM ionomycin for 10 min. The incubation buffer was collected, lucifer yellow was detected by reading the fluorescence, and 3 H-dextran was detected by scintillation counting. The amount of lucifer yellow or 3 H-dextran released in each sample is expressed as a percentage of the total amount of tracer present in control cells. The data represent the average of triplicate determinations ±SD.

Article Snippet: Cultures of primary human fibroblasts (NIGMS; Coriell Institute for Medical Research, Camden, NJ), NRK, J774, IMR-90, L 6 E 9 , and LLC-MK 2 cell lines were grown in DME containing 10% FBS.

Techniques: Incubation, Activity Assay, Control, Fluorescence

Elevation in [Ca 2+ ] i induces secretion of the 31-kD lysosomally processed form of cathepsin D. Rabbit anti–cathepsin D antibodies were used to probe a Western blot containing a lysate and concentrated supernatants of IMR-90 human fibroblasts. (Lane 1 ) total lysate; (lane 2 ) concentrated supernatant of cells treated with PBS for 5 min; (lane 3 ) concentrated supernatant of cells treated with 10 μM ionomycin for 5 min; (lanes 4 and 5 ) concentrated supernatants of SLO-permeabilized cells incubated for 5 min in buffers containing 0 or 1 μM Ca 2+ , respectively. Different ECL exposures were performed for each treatment to allow visualization of the secreted mature 31-kD cathepsin D band.

Journal: The Journal of Cell Biology

Article Title: Lysosomes Behave as Ca 2+ -regulated Exocytic Vesicles in Fibroblasts and Epithelial Cells

doi:

Figure Lengend Snippet: Elevation in [Ca 2+ ] i induces secretion of the 31-kD lysosomally processed form of cathepsin D. Rabbit anti–cathepsin D antibodies were used to probe a Western blot containing a lysate and concentrated supernatants of IMR-90 human fibroblasts. (Lane 1 ) total lysate; (lane 2 ) concentrated supernatant of cells treated with PBS for 5 min; (lane 3 ) concentrated supernatant of cells treated with 10 μM ionomycin for 5 min; (lanes 4 and 5 ) concentrated supernatants of SLO-permeabilized cells incubated for 5 min in buffers containing 0 or 1 μM Ca 2+ , respectively. Different ECL exposures were performed for each treatment to allow visualization of the secreted mature 31-kD cathepsin D band.

Article Snippet: Cultures of primary human fibroblasts (NIGMS; Coriell Institute for Medical Research, Camden, NJ), NRK, J774, IMR-90, L 6 E 9 , and LLC-MK 2 cell lines were grown in DME containing 10% FBS.

Techniques: Western Blot, Incubation

Ca2 + -dependent β-Hexosaminidase Secretion in Different Cell Types

Journal: The Journal of Cell Biology

Article Title: Lysosomes Behave as Ca 2+ -regulated Exocytic Vesicles in Fibroblasts and Epithelial Cells

doi:

Figure Lengend Snippet: Ca2 + -dependent β-Hexosaminidase Secretion in Different Cell Types

Article Snippet: Cultures of primary human fibroblasts (NIGMS; Coriell Institute for Medical Research, Camden, NJ), NRK, J774, IMR-90, L 6 E 9 , and LLC-MK 2 cell lines were grown in DME containing 10% FBS.

Techniques: